NetworkAnalyzer: Computation and Visualization of Topological Parameters and Centrality Measures for Biological Networks
Yassen Assenov, Nadezhda Doncheva, Thomas Lengauer, and Mario Albrecht
Department of Computational Biology and Applied Algorithmics, Max Planck Institute for Informatics, Campus E1.4, 66123 Saarbrücken, Germany
Biological Use Case: Analyse a biological network and discover its topological properties.
Recipe
Cytoscape version: 2.6
Plugins to Load: NetworkAnalyzer (http://med.bioinf.mpi-inf.mpg.de/netanalyzer/), IntActWSClient
GUI steps:
Describe each step (story), the GUI action to take, and probable remarks
Story |
Action |
Remarks |
Load Ataxins network |
import network via IntActWSClient plugin: search for 'Lim' |
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Run NetworkAnalyzer |
Plugins -> Network Analysis -> Analyze Network |
Decide whether your network should be treated as directed or undirected |
Explore network topology |
click on the tabs to see the parameters and their distributions |
Parameters are stored as node and edge attributes |
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change charts (scatter plot/histogram, axes, log scale, colors, zoom…) -> click 'chart settings', 'chart range' |
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fit function to chart -> click 'fit line' or 'fit power law' |
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export diagrams as graphics -> click 'export chart' |
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export data for spread sheets -> click 'export data' |
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Visualize topological parameters |
click 'visualize computed parameters' -> map degree to node size, map clustering coefficient to node color, map edge betweenness to edge color/size |
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Save network statistics |
click 'save statistics' |
network parameters can be re-imported at any time -> network analysis has to be done only once |
Network modifications |
Plugins -> Network Modifications -> Remove Duplicated Edges |
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Plugins -> Network Modifications -> Remove Self-Loops |
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Extract connected components of network |
Plugins -> Network Modifications -> Connected Components. Select a connected component of interest to be displayed as separate network. |
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Data / Session Files: ataxins_network.sif, ataxins.netstats, NetworkAnalyzer_Session.cys
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