Diff for "Cytoscape_User_Manual/ImportingNetworksFromWebServices"

Importing Networks from External Databases

Cytoscape users can import networks and pathways directly from external databases.

By default, direct access to the following external resources is provided:

• [http://www.ebi.ac.uk/intact/site/index.jsf IntAct]: an open source database of protein interaction data, hosted at EMBL-EBI.

• [http://www.pathwaycommons.org Pathway Commons]: an open source portal, providing access to multiple integrated data sets, including: Reactome, IntAct, HPRD, HumanCyc, MINT, the MSKCC Cancer Cell Map, and the NCI/Nature Pathway Interaction database.

Getting Started

To get started, select: File → Import → Network from web services...

attachment:file_import.png

Example #1: Retrieving Protein-Protein Interaction Networks from IntAct

• Select: File → Import → Network from web services...

• From the pull-down menu, select the IntAct Web Service Client.

• Enter a search term, such as BRCA1
• Click the Search button.

attachment:intact_import.png

After confirming the download of interaction data, the network of BRCA1 will be imported and visualized.

Example #2: Retrieving Pathways and Networks from Pathway Commons

• Select: File → Import → Network from web services...

• From the pull-down menu, select the Pathway Commons Service Client.

Then, follow the three-step process outlined below:

• Step 1: Enter your search term; for example: BRCA1
• Step 2: Select the protein or small molecule of interest. Full details regarding each molecule is shown in the bottom left panel.
• Step 3: Download a specific pathway or interaction network.

attachment:3_steps.png

Downloading Pathways and Interaction Networks

In Step 3, you can simply double-click on a pathway of interest, or click on the Interaction Networks tab. The Interaction Networks tab enables you to filter interactions by data source and/or interaction type. For example, you can choose to restrict your network to direct physical interactions from HPRD and MINT only:

attachment:intxn_filter.png

Pathway Commons Options

You can configure access options from the Options tab. There are two retrieval options:

• Simplified Binary Model: Retrieve a simplified binary network, as inferred from the original BioPAX representation. In this representation, nodes within a network refer to physical entities only, and edges refer to inferred interactions.
• Full Model: Retrieve the full model, as stored in the original BioPAX representation. In this representation, nodes within a network can refer to physical entities and interactions.

By default, the simplified binary model is selected.