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When you start Cytoscape, you can access basic functions from the Welcome Screen:
The Welcome Screen is designed to access commonly used features of Cytoscape including:
- Create new network
- Import network
- From file
- From public database
- Import interactome for model organisms
- Open recently used session file
When a network is loaded, Cytoscape will look similar to the image below:
Most functionalities are self-explanatory, but we'll go through a concise explanation for clarity. The main window here has several components:
- The Menu Bar at the top (see below for more information about each menu).
- The Tool Bar, which contains icons for commonly used functions. These functions are also available via the menus. Hover the mouse pointer over an icon and wait momentarily for a description to appear as a tooltip.
- The Network Panel (Network tab of the Control Panel, top left). This contains an optional network overview pane (shown at the bottom left).
- The main Network View Window, which displays the network.
- The Table Panel (bottom right panel), which displays columns of selected nodes and edges and enables you to modify the values of column data.
The Network Panel and Table Panel are dockable tabbed Panels. You can undock any of these panels by clicking on the Float Window control in the upper-right corner of the CytoPanel. This is useful when you want assign the network panel as much screen space as possible. To dock the window again, click the Dock Window icon . Clicking the Hide Panel icon will hide the panel; this can be shown again by choosing View → Show and selecting the relevant panel.
If you click this control, for example on the Table Panel, you will now have two Cytoscape windows, the main window, and a new window labeled Table Panel, similar to the one shown below. A popup will be displayed when you put the mouse pointer on a cell.
Note that Table Panel now has a Dock Window control. If you click this control, the window will dock onto the main window. For more information on the panels in Cytoscape, see the Panels section.
Cytoscape also has an edit functionality that enables you to build and modify networks interactively within the network canvas. To edit a network, just right-click on the open space of network window, select the menu item Add → Node, a new node will be added to the canvas. To add an edge, right click on a node, choose the menu item Edit → Add Edge. Then select the target node, a new edge will be added between the two nodes. In a similar way annotation objects can be added; pictures, shapes or textboxes; much like in MS PowerPoint or similar software. Detailed information on network editing can be found in the Editing Networks section.
The File menu contains most basic file functionality: File → Open for opening a Cytoscape session file; File → New for creating a new network, either blank for editing, or from an existing network; File → Save for saving a session file; File → Import for importing data such as networks and data; and File → Export for exporting data and images. File → Export → Network View as Graphics lets you export the network in either JPEG, PDF, PNG, Post Script or SVG format.
File → Recent Session will list recently opened session files for quick access. File → Run allows you to specify a Cytoscape script file to run, and File → Print Current Network... allows printing.
The Edit menu contains Cut, Copy and Paste functions, as well as Undo and Redo functions which undo and redo edits made in the Table Panel, the Network Editor and to layout.
There are also options for creating and destroying views (graphical representations of a network) and networks (the raw network data – not yet visualized), as well as an option for deleting selected nodes and edges from the current network. All deleted nodes and edges can be restored to the network via Edit → Undo.
There are also other editing options; Remove Duplicated Edges will delete edges that are complete duplicates, keeping one edge, Remove Self-Loops removes edges that have the same source and target node, and Delete Selected Nodes and Edges... deletes a selected subset of the network. Rename Network... allows you to rename the currently selected network.
Editing preferences for properties and apps is found under Edit → Preferences → Properties.... More details on how to edit preferences can be found here.
The View menu allows you to display or hide the Control Panel, Table Panel, Tool Panel and the Result Panel. It also provides the control of other view-related functionality.
The Select menu contains different options for selecting nodes and edges.
The Layout menu has an array of features for visually organizing the network. The features in the top portion of the network (Bundle Edges, Clear Edge Bends, Rotate, Scale, Align and Distribute) are tools for manipulating the network visualization. The bottom section of the menu lists a variety of layout algorithms which automatically lay a network out.
The Apps menu gives you access to the App Manager (Apps → App Manager) for managing (install/update/delete) your apps and may have options added by apps that have been installed. Depending on which apps are loaded, the apps that you see may be different than what appear here. The below picture shows a Cytoscape installation without installed apps.
Note: A list of available Cytoscape apps with descriptions is available online at: http://apps.cytoscape.org
In previous versions of Cytoscape, apps were called plugins and served a similar function.
The Help menu allows you to launch the online help viewer and browse the table of contents for this manual (Contents).
The Citations option displays the main literature citation for Cytoscape, as well as a list of literature citations for installed apps. The list will be different depending on the set of apps you have installed.
The Help menu also allows you to connect directly to Cytoscape Help Desk and the Bug Report interface.
Cytoscape allows multiple networks to be loaded at a time, either with or without a view. A network stores all the nodes and edges that are loaded by the user and a view displays them.
An example where a number of networks have been loaded is shown below:
The network manager (in Control Panel) shows the networks that are loaded. Clicking on a network here will make that view active in the main window, if the view exists. Each network has a name and size (number of nodes and edges), which are shown in the network manager. If a network is loaded from a file, the network name is the name of the file.
Some networks are very large (thousands of nodes and edges) and can take a long time to display. For this reason, a network in Cytoscape may not contain a "view". Networks that have a view are in normal black font and networks that don’t have a view are highlighted in red. You can create or destroy a view for a network by right-clicking the network name in the network manager or by choosing the appropriate option in the Edit menu. You can also destroy previously loaded networks this way.
Certain operations in Cytoscape will create new networks. If a new network is created from an old network, for example by selecting a set of nodes in one network and copying these nodes to a new network (via the File → New → Network option), it will be shown immediately follows the network that it was derived from.
The available network views are also arranged as multiple overlapping windows in the network view window. You can maximize, minimize, and destroy network views by using the normal window controls for your operating system.
Arrange Network Windows
When you work on multiple networks, you can arrange the network view windows under View → Arrange Network Windows.
The Network Overview Window
The network overview window shows an overview (or "bird’s eye view") of the network. It can be used to navigate around a large network view. The blue rectangle indicates the portion of the network currently displayed in the network view window, and it can be dragged with the mouse to view other portions of the network. Zooming in will cause the rectangle to appear smaller and vice versa.