ClusterMaker

John 'Scooter' Morris

University of California, San Francisco

Biological Use Case: Find possible complexes, protein families, functional relationships and view in biological context.

Recipe

Cytoscape version: Version number (2.6)

Plugins to Load: ClusterMaker (from what URL?)

GUI steps:

Describe each step (story), the GUI action to take, and probable remarks

Story

Action

Remarks

Start with expression data for studies into mechanism for galactose utilization.

open galfiltered.cys session

galfilterd may be replaced by time series data from Nathan Salomonis, AllanK will follow up on this

run clustering to determine interesting subnets

select plugins -> cluster -> hierarchical cluster. For settings, use maximal linkage, cluster attributes as well as nodes, cluster on just expression data

visualize and navigate the clusters

on treeview window, explore by clicking on points on the dendogram

shows Eisen treeview visualization. Clicking/selecting a particular row in the heatmap will result in the expression values for that column being overlaid on the network view

use shift-drag to draw a box and see results on network

use shift-click to pick individual columns

Data Files: Attach data files

Presentation: Attach your presentation

Webstart: Attach a webstart

Video: Attach a video link

Funding for Cytoscape is provided by a federal grant from the U.S. National Institute of General Medical Sciences (NIGMS) of the Na tional Institutes of Health (NIH) under award number GM070743-01. Corporate funding is provided through a contract from Unilever PLC.

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