ClusterMaker
John 'Scooter' Morris
University of California, San Francisco
Biological Use Case: Find possible complexes, protein families, functional relationships and view in biological context.
Recipe
Cytoscape version: Version number (2.6)
Plugins to Load: ClusterMaker (from what URL?)
GUI steps:
Describe each step (story), the GUI action to take, and probable remarks
Story |
Action |
Remarks |
Start with expression data for studies into mechanism for galactose utilization. |
open galfiltered.cys session |
galfiltered may be replaced by time series data from Nathan Salomonis, AllanK will follow up on this |
run clustering to determine interesting subnets |
select plugins -> cluster -> hierarchical cluster. For settings, use maximal linkage, cluster attributes as well as nodes, cluster on just expression data |
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visualize and navigate the clusters |
on treeview window, explore by clicking on points on the dendogram. Press 'map colors onto network' diagram |
shows Eisen treeview visualization. Clicking/selecting a particular row in the heatmap will result in the expression values for that column being overlaid on the network view |
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adjust contrast by settings -> contrast |
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use shift-drag to draw a box and see results on network |
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use shift-click to pick individual columns |
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select an individual row by clicking on it |
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animate expression values over time |
select 'options'. On popup, click on specific attributes to select. Optionally order attributes. Select all attributes and click on 'animate'. |
will show nicely if we have time series data. TODO: AllanK check with Nathan on this |
Data Files: Attach data files
Presentation: Attach your presentation
Webstart: Attach a webstart
Video: Attach a video link